Interstate 64 (I-64) is an Interstate Highway in the Eastern United States. Its western terminus is at I-70, U.S. Route 40 (US 40), and US 61 in Wentzville, Missouri. Its eastern terminus is at an interchange with I-264 and I-664 at Bowers Hill in Chesapeake, Virginia. I-64 connects the major metropolitan areas of St. Louis, Missouri; Louisville and Lexington in Kentucky; Charleston, West Virginia; and Richmond and Hampton Roads in Virginia. At 953.74 miles, I-64 is the second longest interstate highway not ending with a 5 or 0, after I-94.
I-64 overlaps with I-55, I-57, I-75, I-77, I-81, and I-95. I-64 does not maintain exit number continuity for any of the overlaps, as each of the six north-south routes maintain their exit numbering on their respective overlaps with I-64.